Sponsoring Institution
National Institute of Food and Agriculture
Status
COMPLETE
Funding Source
HATCH
Division
NIFA Formula
Reporting Frequency
Annual
Project Director
Killiny, Nabil
Accession Number
1008683
Project Number
FLA-CRC-005485
Dates
2016-04-28 - 2020-10-31
Animal Health Component
35%
Performing Department
Citrus Research and Education Center, Lake Alfred
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Keywords
biomarker
digital citrus metabolite database
huanglongbing
metabolome
proteome
Research Effort
Applied (35%)
Basic (50%)
Developmental (15%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 212 - Pathogens and Nematodes Affecting Plants |
920 - Orange |
1160 - Pathology |
100% |
Non-technical Summary
In this project, we try to find a biomarker that can be used for early detection of Huanglongbing in citrus.
Goals / Objectives
Identification of potential biomarkers and other tools for the early detection of Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus greening (Huanglongbing) using trans-disciplinary, time-wise study. The study includes simultaneous GC-MS and HPLC-MS metabolite profiling of citrus tissues (leaf, phloem sap, root tissue), analysis of phytohormone levels, ans protein expression.
Methods (unparsed)
Metabolome (GC-MS, HPLC), proteomics (2D-electrophoresis and LC-MS-MS), and digitaldatabase.
Methods
Metabolome (GC-MS, HPLC), proteomics (2D-electrophoresis and LC-MS-MS), and digitaldatabase.