Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
DUAN Y
Accession Number
439496
Project Number
6034-22000-045-006R
Dates
2020-10-01 - 2025-08-31
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
219 SOUTH ROCK ROAD
FT PIERCE,FL 34945
Keywords
(clas)
asiaticus
candidatus
citrus
cultivars
hlb
hybrids
liberibacter
resistance/tolerance
scion
titers
Research Effort
Applied (30%)
Basic (60%)
Developmental (10%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 212 - Pathogens and Nematodes Affecting Plants |
910 - Grapefruit |
1040 - Molecular biology |
50% |
| 212 - Pathogens and Nematodes Affecting Plants |
920 - Orange |
1060 - Biology (whole systems) |
50% |
Goals / Objectives
Rapid screening the advanced hybrids for resistance to CLas under greenhouse conditions to facilitate selection of hybrids with broad spectrum of resistance against multiple CLas isolates.
Methods (unparsed)
By participating in the concerted, parallel and coordinated efforts towards developing new HLB resistant/tolerant scion cultivars, ARS will propagate the advanced hybrids on common rootstock, sour orange and/or US 942, and then evaluate their resistance/tolerance against at least two distinct CLas isolates using graft-based assay. The disease index and CLas titers will be determined via qPCR and other measurements.
Methods
By participating in the concerted, parallel and coordinated efforts towards developing new HLB resistant/tolerant scion cultivars, ARS will propagate the advanced hybrids on common rootstock, sour orange and/or US 942, and then evaluate their resistance/tolerance against at least two distinct CLas isolates using graft-based assay. The disease index and CLas titers will be determined via qPCR and other measurements.