Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
ROSSKOPF E N
Accession Number
438496
Project Number
6034-21000-019-005R
Dates
2019-10-01 - 2022-01-31
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
219 SOUTH ROCK ROAD
FT PIERCE,FL 34945
Keywords
antimicrobial
asiaticus
candidatus
citrus
greening
huanglongbing
liberibacter
peptide
prevention
therapy
Research Effort
Applied (30%)
Basic (70%)
Developmental (0%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants |
920 - Orange |
1000 - Biochemistry and biophysics |
50% |
| 204 - Plant Product Quality and Utility (Preharvest) |
999 - Citrus, general/other |
1080 - Genetics (excludes breeding) |
50% |
Goals / Objectives
- Identify the most effective antimicrobial peptide mixtures and delivery methods for curing HLB-infected trees.
- Characterize immune-priming effects of antimicrobial peptides and quantify antimicrobial peptides capacity to vaccinate healthy trees against Candidatus Liberibacter Asiaticus (Clas) and protect against Asian citrus psyllid feeding.
- Integrate research outputs with extension and outreach efforts. Communicate findings to growers and establish connections to facilitate future antimicrobial peptide (AP) adoption.
- Perform an economic analysis and register AP products with the Environmental Protection Agency (EPA).
Methods (unparsed)
We propose to develop effective therapies and protectants using antimicrobial peptide solutions and evaluate the cost-effective application methods to HLB-positive trees or healthy young trees for Candidatus Liberibacter Asiaticus (CLas) control. Various cocktails will be formulated and different delivery methods will be tested in the greenhouse and field. The cocktail and delivery method proven most effective will be introduced to growers for field evaluations. The citrus tristeza virus (CTV)-mediated expression delivery method will be also included for testing.
Methods
We propose to develop effective therapies and protectants using antimicrobial peptide solutions and evaluate the cost-effective application methods to HLB-positive trees or healthy young trees for Candidatus Liberibacter Asiaticus (CLas) control. Various cocktails will be formulated and different delivery methods will be tested in the greenhouse and field. The cocktail and delivery method proven most effective will be introduced to growers for field evaluations. The citrus tristeza virus (CTV)-mediated expression delivery method will be also included for testing.