Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
THOMSON J G
Accession Number
437389
Project Number
2030-21220-003-003R
Dates
2019-10-01 - 2024-05-31
Recipient Organization
WESTERN REGIONAL RES CENTER
ALBANY,CA 94710
Keywords
(hlb)
citrus
gaantry
huanglongbing
resistance
tissue
transformation
Research Effort
Applied (25%)
Basic (75%)
Developmental (0%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 201 - Plant Genome, Genetics, and Genetic Mechanisms |
999 - Citrus, general/other |
1040 - Molecular biology |
100% |
Goals / Objectives
- Develop transformation vectors for pattern-activated molecular pattern (PAMP) immune response resistance genes expressing transgenic Huanglongbing (HLB) resistant citrus
- Stack the PAMPs into a T-DNA for transformation and defense response testing.
Methods (unparsed)
Transformation constructs will be designed to introduce pattern-activated molecular pattern (PAMP) immune response resistance genes to trigger native plant defense mechanisms to Huanglongbing (HLB) infection. Using the Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technologY (GAANTRY) system multiple genes will be stacked into a single T-DNA for delivery. These modified Agrobacterium containing T-DNAs will be shared to members of this grant for citrus transformation. Plants produced will be testing by a rapid response native defense system readout at UC Davis. Multiple stacked PAMP T-DNAâ¿¿s will be examined. Once defense activation has been demonstrated with the potential disease resistance the PAMP T-DNA stacks will be introduced into various citrus cultivars for direct HLB testing. The resulting citrus trees will contain citrus-derived candidate resistance genes and the capacity for recombinase-mediated gene stacking.
Methods
Transformation constructs will be designed to introduce pattern-activated molecular pattern (PAMP) immune response resistance genes to trigger native plant defense mechanisms to Huanglongbing (HLB) infection. Using the Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technologY (GAANTRY) system multiple genes will be stacked into a single T-DNA for delivery. These modified Agrobacterium containing T-DNAs will be shared to members of this grant for citrus transformation. Plants produced will be testing by a rapid response native defense system readout at UC Davis. Multiple stacked PAMP T-DNA's will be examined. Once defense activation has been demonstrated with the potential disease resistance the PAMP T-DNA stacks will be introduced into various citrus cultivars for direct HLB testing. The resulting citrus trees will contain citrus-derived candidate resistance genes and the capacity for recombinase-mediated gene stacking.