Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
NIEDZ R P
Accession Number
435920
Project Number
6034-21000-018-016R
Dates
2018-11-01 - 2021-10-31
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
219 SOUTH ROCK ROAD
FT PIERCE,FL 34945
Keywords
&
chemistry
citrus
development
disease
fenton
ferrous
foundation
grant
greening
h202
hlb
huanglongbing
iron
mineral
nutrition
proposal
reaction
research
Research Effort
Applied (50%)
Basic (50%)
Developmental (0%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 204 - Plant Product Quality and Utility (Preharvest) |
920 - Orange |
1080 - Genetics (excludes breeding) |
40% |
| 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants |
910 - Grapefruit |
1000 - Biochemistry and biophysics |
30% |
| 212 - Pathogens and Nematodes Affecting Plants |
999 - Citrus, general/other |
1160 - Pathology |
30% |
Goals / Objectives
The overall objective is to determine if the use of iron Fe2+ and an organic acid will cure or substantially reduce Candidatus Liberibacter asiaticus titer in citrus trees with Huanglongbing.
- Determine the effect of the ratio and concentration of iron Fe2+ + organic acid on hydroxyl radical production and stability.
- Determine the phytotoxic levels of iron Fe2+ + organic acid solutions on citrus.
- Determine the effect of iron Fe2+ + organic acid solutions on huanglongbing (HLB)titer using a rapid greenhouse, huanglonbing (HLB)-infected citron, rooted shoot bud assay.
- Determine the effect of iron Fe2+ + organic acid solutions on protecting healthy citrus from becoming infected by huanglongbing (HLB)+ psyllids.
- Determine the main and interactive effects of supplemental iron, manganese, boron, zinc, and copper using a rapid greenhouse, huanglongbing (HLB)-infected citron, rooted shoot bud assay. Iron delivered as Fe2+ + organic acid.
- Determine the effect of iron Fe2+ + organic acid solutions on mature huanglongbing (HLB)-infected field trees.
- Determine the effect of iron Fe2+ + organic acid solutions on nearly planted ( Project Methods Laboratory, greenhouse, and field experiments will be conducted.
Laboratory experiments will determine the stability of the iron Fe(2+) ion with various proportions and concentrations of citric acid, the production of hydroxyl radicals by the Fenton reaction, and the proportion of Fe(2+) iron with various proportions and concentrations of citric acid and pH. Greenhouse experiments will use a model system of huanglongbing (HLB)-infected citron cuttings to evaluate the effect of various proportions and concentrations of iron Fe(2+) and citric acid on huanglongbing (HLB) titer and plant growth, as well as various types of applications (foliar, drench, foliar + drench). Field experiments will determine the effect of iron Fe(2+) + citric acid applied as foliar, drench, or foliar + drench on the health and HLB-titer of mature and young citrus trees HLB-infected citron cuttings to evaluate the effect of various proportions and concentrations of iron Fe(2+) and citric acid.
Methods (unparsed)
Laboratory, greenhouse, and field experiments will be conducted. Laboratory experiments will determine the stability of the iron Fe(2+) ion with various proportions and concentrations of citric acid, the production of hydroxyl radicals by the Fenton reaction, and the proportion of Fe(2+) iron with various proportions and concentrations of citric acid and pH. Greenhouse experiments will use a model system of huanglongbing (HLB)-infected citron cuttings to evaluate the effect of various proportions and concentrations of iron Fe(2+) and citric acid on huanglongbing (HLB) titer and plant growth, as well as various types of applications (foliar, drench, foliar + drench). Field experiments will determine the effect of iron Fe(2+) + citric acid applied as foliar, drench, or foliar + drench on the health and HLB-titer of mature and young citrus trees HLB-infected citron cuttings to evaluate the effect of various proportions and concentrations of iron Fe(2+) and citric acid.
Methods
Laboratory, greenhouse, and field experiments will be conducted. Laboratory experiments will determine the stability of the iron Fe(2+) ion with various proportions and concentrations of citric acid, the production of hydroxyl radicals by the Fenton reaction, and the proportion of Fe(2+) iron with various proportions and concentrations of citric acid and pH. Greenhouse experiments will use a model system of huanglongbing (HLB)-infected citron cuttings to evaluate the effect of various proportions and concentrations of iron Fe(2+) and citric acid on huanglongbing (HLB) titer and plant growth, as well as various types of applications (foliar, drench, foliar + drench). Field experiments will determine the effect of iron Fe(2+) + citric acid applied as foliar, drench, or foliar + drench on the health and HLB-titer of mature and young citrus trees HLB-infected citron cuttings to evaluate the effect of various proportions and concentrations of iron Fe(2+) and citric acid.