Grant Information

DEVELOPMENT OF A SYNTHETIC LIBERIBACTER AS A CROSS-PROTECTIVE STRAIN AGAINST HLB

Sponsoring Institution Agricultural Research Service/USDA
Status ACTIVE
Funding Source USDA COOPERATIVE AGREEMENT
Reporting Frequency Annual
Project Director SHATTERS R G
Accession Number 434554
Project Number 6034-22320-004-039S
Dates 2018-04-01 - 2020-01-29
Recipient Organization UNIVERSITY OF FLORIDA

FORT PIERCE,FL 33450
Keywords citrus
greening
hlb
huanglongbing
psyllid
Research Effort Applied (30%)
Basic (45%)
Developmental (25%)
Classification Parameters
Knowledge AreaSubject of InvestigationField of SciencePercent
211 - Insects, Mites, and Other Arthropods Affecting Plants 910 - Grapefruit 1040 - Molecular biology 40%
215 - Biological Control of Pests Affecting Plants 920 - Orange 1130 - Entomology and acarology 40%
211 - Insects, Mites, and Other Arthropods Affecting Plants 1131 - Wine grapes 1130 - Entomology and acarology 10%
216 - Integrated Pest Management Systems 1130 - Table grapes 1040 - Molecular biology 10%
Goals / Objectives
To conduct collaborative research on the testing of synthetically derived strains from Candidatus Liberibacter asiaticus to develop a non-pathogenic strain that can out-compete the pathogenic strain within citrus trees.
Methods (unparsed)

Collaboration with the University of Florida is necessary for two reasons: 1) Utilize expertise in plant pathology and plant pathogen effector biology; and 2) to obtain access through this collaboration to University of Florida containment facility that is required for initial evaluation of synthetic Liberibacter strains. Research involves initial interactions with Synthetic Biology, Inc. A company with proprietary methods used to develop synthetic bacteria. They will provide the synthetic bacteria for testing for replication and within citrus and Asian citrus psyllids and for evaluation fo the synthetic bacterium¿s ability to induce huanglongbing (HLB) symptoms in the plant and to out-compete pathogenic strains of Candidatus Liberibacter asiaticus that is also within the citrus plant. Most experiments will be conducted in the containment greenhouses located on University of Florida propter at the Indian River Research and Education Center in Fort Pierce, Florida. Plant and insect tissue samples will be transferred to the USDA, U.S. Horticultural Research laboratory in Fort Pierce, Florida, for molecular analysis.