Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA COOPERATIVE AGREEMENT
Reporting Frequency
Annual
Project Director
BAI J
Accession Number
434437
Project Number
6034-41430-007-006A
Dates
2018-01-15 - 2023-01-14
Recipient Organization
UNIVERSITY OF CALIFORNIA, RIVERSIDE
RIVERSIDE,CA 92521
Keywords
citrus
disease
flavor
huanglongbing
mandarin
markers
molecular
orange
poncirus
resistance
Research Effort
Applied (40%)
Basic (40%)
Developmental (20%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 502 - New and Improved Food Products |
999 - Citrus, general/other |
1000 - Biochemistry and biophysics |
58% |
| 502 - New and Improved Food Products |
1129 - Berries and cane fruits, general/other |
1160 - Pathology |
16% |
| 503 - Quality Maintenance in Storing and Marketing Food Products |
1469 - Solanaceous and related crops, general/other (for potato use 1310) |
2000 - Chemistry |
16% |
| 503 - Quality Maintenance in Storing and Marketing Food Products |
1099 - Tropical/subtropical fruit, general/other |
1020 - Physiology |
10% |
Goals / Objectives
Determine Huanglongbing (HLB) resistant/tolerant genes in citrus using qualitative trait loci mapping and validate resistance/tolerant markers especially in manderin- and Poncirus-derived plantings. Identify field HLB-resistant/tolerant hybrids in the University of California Breeding program, from selection growing in Florida with help of ARS, and select promising candidates for evaluation of flavor quality by USDA-ARS.
Methods (unparsed)
Conduct genotyping-by-seqencing and genotyping with single nucleotide polymorphism (SNP) markers in parents and hybrids focusing on mandarin- and Poncirus-derived hybrids. Conduct pNOME approach (sequencing pooled samples of tolerant and susceptible genotypes). Phenotype UCR breeding program citrus lines grown in Florida with help of ARS by determining tree growth, health index, pathogen bacterial titer and disease symptoms.
Methods
Conduct genotyping-by-seqencing and genotyping with single nucleotide polymorphism (SNP) markers in parents and hybrids focusing on mandarin- and Poncirus-derived hybrids. Conduct pNOME approach (sequencing pooled samples of tolerant and susceptible genotypes). Phenotype UCR breeding program citrus lines grown in Florida with help of ARS by determining tree growth, health index, pathogen bacterial titer and disease symptoms.