Grant Information

EXOTIC PATHOGENS OF CITRUS: CURATION, DIAGNOSTICS, AND INTERACTIONS

Sponsoring Institution Agricultural Research Service/USDA
Status COMPLETE
Funding Source USDA INHOUSE
Reporting Frequency Annual
Project Director HARTUNG J S
Accession Number 432646
Project Number 8042-22000-301-000D
Dates 2017-04-09 - 2022-03-16
Recipient Organization AGRICULTURAL RESEARCH SERVICE
RM 331, BLDG 003, BARC-W
BELTSVILLE,MD 20705-2351
Keywords antibodies
asiaticus
canary
candidatus
citrus
exotic
grapefruit
green
huanglongbing
liberibacter
orange
pathogens
scfv
sweet
tristeza
virus
Research Effort Applied (20%)
Basic (80%)
Developmental (0%)
Classification Parameters
Knowledge AreaSubject of InvestigationField of SciencePercent
212 - Pathogens and Nematodes Affecting Plants 920 - Orange 1160 - Pathology 40%
212 - Pathogens and Nematodes Affecting Plants 999 - Citrus, general/other 1160 - Pathology 40%
212 - Pathogens and Nematodes Affecting Plants 910 - Grapefruit 1160 - Pathology 20%
Goals / Objectives
  1. Evaluate patterns of gene expression in mixed infections between CTV and HLB in citrus, with the goal of discovering both cross-protecting strains and potential synergistic interactions.
  2. Develop new diagnostic reagents for emerging citrus pathogens and evaluate them as research reagents for the citrus community.
Methods (unparsed)

Host gene expression with two phloem limited pathogens - We intend to define citrus genes expressed in response to infection by phloem limited pathogens Citrus tristeza virus (CTV) and 'Ca. Liberibacter asiaticus' (CaLas). RNA Seq: Asymptomatic, young leaf tissue will be ground to a powder in liquid nitrogen and RNA will be isolated using a Trizol protocol. RNA will be used for RNA sequencing (Illumina 2500). Paired-end reads will be mapped to Citrus sinensis â¿¿Valenciaâ¿¿ reference genome. Differentially expressed transcripts will be identified. P

Methods
Host gene expression with two phloem limited pathogens - We intend to define citrus genes expressed in response to infection by phloem limited pathogens Citrus tristeza virus (CTV) and 'Ca. Liberibacter asiaticus' (CaLas). RNA Seq: Asymptomatic, young leaf tissue will be ground to a powder in liquid nitrogen and RNA will be isolated using a Trizol protocol. RNA will be used for RNA sequencing (Illumina 2500). Paired-end reads will be mapped to Citrus sinensis 'Valencia' reference genome. Differentially expressed transcripts will be identified. P
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Outputs

Objective 1: Evaluate patterns of gene expression in mixed infections between CTV and HLB in citrus, with the goal of discovering both cross- protecting strains and potential synergistic interactions. Objective 2: Develop new diagnostic reagents for emerging citrus pathogens and evaluate them as research reagents for the citrus community. Host gene expression with two phloem limited pathogens - We intend to define citrus genes expressed in response to infection by phloem limited pathogens Citrus tristeza virus (CTV) and 'Ca. Liberibacter asiaticus' (CaLas). RNA Seq: Asymptomatic, young leaf tissue will be ground to a powder in liquid nitrogen and RNA will be isolated using a Trizol protocol. RNA will be used for RNA sequencing (Illumina 2500). Paired- end reads will be mapped to Citrus sinensis â¿¿Valenciaâ¿¿ reference genome. Differentially expressed transcripts will be identified. P

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