Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
YOKOMI R K
Accession Number
431214
Project Number
2034-22000-013-06T
Dates
2017-01-01 - 2020-02-25
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
PARLIER,CA 93648
Keywords
(hlb)
clas
ddpcr
huanglongbing
qpcr
Research Effort
Applied (40%)
Basic (50%)
Developmental (10%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 212 - Pathogens and Nematodes Affecting Plants |
920 - Orange |
1160 - Pathology |
75% |
| 212 - Pathogens and Nematodes Affecting Plants |
930 - Lemon |
1170 - Epidemiology |
25% |
Goals / Objectives
The research objective is to improve reliable diagnosis of "Candidatus Liberibacter asiaticus", presumptive causal agent of huanglongbing (HLB)in the U.S.
Methods (unparsed)
The approach includes deep sequencing using MiSeq Illumina to improve full length sequence data of 'Ca. Liberibacter asiaticus'; and bioinformatic analysis, employing primer design software, Taqman probes, real time quantitative PCR, droplet digital PCR, high throughput robotic DNA extraction and purification of nucleic acid.
Methods
The approach includes deep sequencing using MiSeq Illumina to improve full length sequence data of 'Ca. Liberibacter asiaticus'; and bioinformatic analysis, employing primer design software, Taqman probes, real time quantitative PCR, droplet digital PCR, high throughput robotic DNA extraction and purification of nucleic acid.