Grant Information

IMPROVING EARLY DETECTION OF HLB VIA ACP NYMPH/CITRUS FLUSH SAMPLING

Sponsoring Institution Agricultural Research Service/USDA
Status ACTIVE
Funding Source USDA COOPERATIVE AGREEMENT
Reporting Frequency Annual
Project Director MCCOLLUM T G
Accession Number 430899
Project Number 6034-21000-016-13S
Dates 2016-02-24 - 2017-08-30
Recipient Organization TEXAS A&M UNIVERSITY - KINGSVILLE CITRUS CENTER
312 N. INTERNATIONAL BLVD.
WESLACO,TX 78596
Keywords citrus
huanglongbing (hlb) asian citrus psyllid cadidatus liberibacter asiaticus quantitative pcr
Research Effort Applied (30%)
Basic (70%)
Developmental (0%)
Classification Parameters
Knowledge AreaSubject of InvestigationField of SciencePercent
203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants 920 - Orange 1000 - Biochemistry and biophysics 50%
204 - Plant Product Quality and Utility (Preharvest) 999 - Citrus, general/other 1080 - Genetics (excludes breeding) 50%
Goals / Objectives
Provide data confirming the value of sampling citrus flush infested with Asian citrus psyllid (ACP) nymphs for CLas diagnostics.
Methods (unparsed)

Citrus flush will be enclosed with 10 Asian citrus psyllids from a Candidatus Liberibacter asiaticus infected colony for 10 day followed by Asian citrus psyllid (ACP) removal. ACP adults will be assayed for CLas infection at the time of removal. ACP nymphs will be allowd to develop from eggs laid that were laid during exposure of the flush. When nymphs have reached the third instar, they will be collected along with the infested shoots and assayed for CLas. Plants will subsequently be held free of ACP and assayed for CLas at intervals throughout the duration of the project. Trees will also be evaluated for HLB symptoms following removal of ACP.