Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
HALL D G
Accession Number
429283
Project Number
6034-22320-004-19R
Dates
2015-08-01 - 2018-12-31
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
219 SOUTH ROCK ROAD
FT PIERCE,FL 34945
Keywords
&
asian
citrus
development
foundation
greening
huanglongbing
psyllid
research
Research Effort
Applied (30%)
Basic (45%)
Developmental (25%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 211 - Insects, Mites, and Other Arthropods Affecting Plants |
910 - Grapefruit |
1040 - Molecular biology |
40% |
| 215 - Biological Control of Pests Affecting Plants |
920 - Orange |
1130 - Entomology and acarology |
40% |
| 211 - Insects, Mites, and Other Arthropods Affecting Plants |
1131 - Wine grapes |
1130 - Entomology and acarology |
10% |
| 216 - Integrated Pest Management Systems |
1130 - Table grapes |
1040 - Molecular biology |
10% |
Goals / Objectives
To inoculate citrus germplasm with the bacteria responsible for citrus huanglongbing (HLB) disease in order to evaluate germplasm for resistance to the disease.
Methods (unparsed)
USDA-ARS-U.S. Horticultural Research Laboratory, Fort Pierce Florida, is producing thousands of citrus scion or rootstock plants transformed to express peptides that might mitigate citrus greening disease; additional plants are being continually produced. The more rapidly this germplasm can be evaluated, the sooner we will be able to identify transgenic strategies for controlling the disease. The purpose of this proposal is to support a high-throughput inoculation program for the purposes of infecting and evaluating transgenic citrus for hunaglongbing resistance. Briefly, individual plants are caged with infected psyllids for two weeks, and then housed for six months in a greenhouse with an open infestation of infected psyllids. Plants are then moved into a psyllid-free greenhouse and evaluated for growth, huanglongbing symptoms and Liberibacter asia ticus titer, and later to the field for further evaluations. The funds requested will be for two technicians dedicated to the project, primers and associated supplies for PCR, and general supplies including insect cages.
Methods
USDA-ARS-U.S. Horticultural Research Laboratory, Fort Pierce Florida, is producing thousands of citrus scion or rootstock plants transformed to express peptides that might mitigate citrus greening disease; additional plants are being continually produced. The more rapidly this germplasm can be evaluated, the sooner we will be able to identify transgenic strategies for controlling the disease. The purpose of this proposal is to support a high-throughput inoculation program for the purposes of infecting and evaluating transgenic citrus for hunaglongbing resistance. Briefly, individual plants are caged with infected psyllids for two weeks, and then housed for six months in a greenhouse with an open infestation of infected psyllids. Plants are then moved into a psyllid-free greenhouse and evaluated for growth, huanglongbing symptoms and Liberibacter asia ticus titer, and later to the field for further evaluations. The funds requested will be for two technicians dedicated to the project, primers and associated supplies for PCR, and general supplies including insect cages.