Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
HALL D G
Accession Number
427881
Project Number
6034-22320-001-52T
Dates
2014-10-01 - 2015-09-30
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
219 SOUTH ROCK ROAD
FT PIERCE,FL 34945
Keywords
asian
citrus
greening
huanglongbing
liberibacter
pysllid
Research Effort
Applied (30%)
Basic (45%)
Developmental (25%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 211 - Insects, Mites, and Other Arthropods Affecting Plants |
910 - Grapefruit |
1040 - Molecular biology |
40% |
| 215 - Biological Control of Pests Affecting Plants |
920 - Orange |
1130 - Entomology and acarology |
40% |
| 211 - Insects, Mites, and Other Arthropods Affecting Plants |
1131 - Wine grapes |
1130 - Entomology and acarology |
10% |
| 216 - Integrated Pest Management Systems |
1130 - Table grapes |
1040 - Molecular biology |
10% |
Goals / Objectives
Screen progeny plants from new citrus crosses for resistance to citrus greening disease.
Methods (unparsed)
Seeds from crosses between citrus and citrus relatives will be planted in Fort Pierce. When the plants are about six months old, they will be challenged with psyllids carrying the bacterial pathogen responsible for citrus greening disease. After a two-week challenge, the plants will be treated with pesticide and the experiment will be continued in the screened enclosures. The plants will subsequently be observed on a regular basis for huanglongbing symptoms and tested for Las by qPCR. The intent is to discover genetic material that is tolerant or resistant to citrus greening disease.
Methods
Seeds from crosses between citrus and citrus relatives will be planted in Fort Pierce. When the plants are about six months old, they will be challenged with psyllids carrying the bacterial pathogen responsible for citrus greening disease. After a two-week challenge, the plants will be treated with pesticide and the experiment will be continued in the screened enclosures. The plants will subsequently be observed on a regular basis for huanglongbing symptoms and tested for Las by qPCR. The intent is to discover genetic material that is tolerant or resistant to citrus greening disease.