Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
DUAN Y
Accession Number
426066
Project Number
6034-22000-039-47R
Dates
2013-11-22 - 2014-06-30
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
219 SOUTH ROCK ROAD
FT PIERCE,FL 34945
Keywords
cbs
citrus
hlb
ornamentals
pcr
ppv
strawberry
thermo
vegetables
viruses
Research Effort
Applied (30%)
Basic (60%)
Developmental (10%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
| 212 - Pathogens and Nematodes Affecting Plants |
910 - Grapefruit |
1040 - Molecular biology |
25% |
| 212 - Pathogens and Nematodes Affecting Plants |
920 - Orange |
1060 - Biology (whole systems) |
25% |
| 212 - Pathogens and Nematodes Affecting Plants |
1114 - Peach |
1040 - Molecular biology |
10% |
| 212 - Pathogens and Nematodes Affecting Plants |
1122 - Strawberry |
1060 - Biology (whole systems) |
10% |
| 212 - Pathogens and Nematodes Affecting Plants |
1429 - Cucurbits, other (includes pumpkin, squash, gourd) |
1040 - Molecular biology |
10% |
| 212 - Pathogens and Nematodes Affecting Plants |
1460 - Tomato |
1060 - Biology (whole systems) |
10% |
| 212 - Pathogens and Nematodes Affecting Plants |
2110 - Ornamental trees and shrubs |
1040 - Molecular biology |
10% |
Goals / Objectives
- Conduct larger-scale field trials with three different locations along with different citrus varieties (a total of 180 trees) to evaluate the thermal effects on Las bacterial population dynamics, plant growth and productivity.
- Develop a mathematical model to predict the time duration required for the best outcome, which will use the data generated from previous and future trials.
Methods (unparsed)
The heat treatment will be conducted in the fields using portable greenhouse. We will use real-time PCR (Polymerase Chain Reaction) to quantify the bacterial titers before and after heat treatment, and measure yields (productivity) of treated and non-treated citrus plants.
Methods
The heat treatment will be conducted in the fields using portable greenhouse. We will use real-time PCR (Polymerase Chain Reaction) to quantify the bacterial titers before and after heat treatment, and measure yields (productivity) of treated and non-treated citrus plants.