Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
STOVER E W
Accession Number
424907
Project Number
6034-21000-014-18R
Dates
2012-04-01 - 2015-03-31
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
219 SOUTH ROCK ROAD
FT PIERCE,FL 34945
Keywords
citrus
huanglongbing
Research Effort
Applied (50%)
Basic (50%)
Developmental (0%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 204 - Plant Product Quality and Utility (Preharvest) |
920 - Orange |
1080 - Genetics (excludes breeding) |
40% |
| 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants |
910 - Grapefruit |
1000 - Biochemistry and biophysics |
30% |
| 212 - Pathogens and Nematodes Affecting Plants |
999 - Citrus, general/other |
1160 - Pathology |
30% |
Goals / Objectives
To transform citrus with genes encoding selected scFv and evaluate the transgenic citrus for resistance to huanglongbing (HLB).
Methods (unparsed)
ARS USHRL Fort Pierce will use these constructs to transform citrus, identify transformed citrus, produce and propagate transformed trees, and screen them for resistance to 'Ca. Liberibacter asiaticus'. ARS U.S. Hortictural Research Laboratory, Ft. Pierce has developed efficient methods for large scale transformation, regeneration and screening of citrus for resistance to Huanglongbing (HLB). ARS Beltsville has developed an array of scFv that bind to critical epitopes on the pathogen which causes huanglongbing in citrus.
Methods
ARS USHRL Fort Pierce will use these constructs to transform citrus, identify transformed citrus, produce and propagate transformed trees, and screen them for resistance to 'Ca. Liberibacter asiaticus'. ARS U.S. Hortictural Research Laboratory, Ft. Pierce has developed efficient methods for large scale transformation, regeneration and screening of citrus for resistance to Huanglongbing (HLB). ARS Beltsville has developed an array of scFv that bind to critical epitopes on the pathogen which causes huanglongbing in citrus.