Sponsoring Institution
Agricultural Research Service/USDA
Status
ACTIVE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Project Director
HARTUNG J S
Accession Number
424255
Project Number
8042-22000-281-16R
Dates
2012-10-01 - 2017-08-31
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
RM 331, BLDG 003, BARC-W
BELTSVILLE,MD 20705-2351
Keywords
huanglongbing
Research Effort
Applied (20%)
Basic (80%)
Developmental (0%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 212 - Pathogens and Nematodes Affecting Plants |
920 - Orange |
1160 - Pathology |
40% |
| 212 - Pathogens and Nematodes Affecting Plants |
999 - Citrus, general/other |
1160 - Pathology |
40% |
| 212 - Pathogens and Nematodes Affecting Plants |
910 - Grapefruit |
1160 - Pathology |
20% |
Goals / Objectives
The overall objectives of this project are:
- to develop a genetically modified citrus psyllid, Diaphorina citri, that is not capable of acquiring and transmitting the pathogen that causes citrus huanglongbing disease
- develop a means to introduce this psyllid into Florida to replace the established population of Diaphorina citri.
pressed in recombinant bacteriophage. The COOPERATORS have the expertise and infrastructure to use these antibodies to identify targets in the insect vector essential for acquisition of 'Ca. Liberibacter asiaticus' by the insect as a prerequisite for the insect becoming an infectious vector of 'Ca. Liberibacter asiaticus' leading to huanglongbing disease.
Methods (unparsed)
ARS (Beltsville) will screen the scFv library against proteins identified by the consortium as likely to be required for acquisition of ¿Ca. Liberibacter asiaticus¿ by Diaphorina citri. Targeted proteins will be selected jointly by ARS Beltsville and other members of the consortium. Selected recombinant antibodies will be provided to cooperators. Genes encoding the recombinant antibodies will also be available as needed. The Cooperator will identify candidate psyllid encoded proteins for screening and use the recombinant antibodies to test for inhibition of acquisition/transmission of the pathogen by Diaphorina citri.
Methods
ARS (Beltsville) will screen the scFv library against proteins identified by the consortium as likely to be required for acquisition of 'Ca. Liberibacter asiaticus' by Diaphorina citri. Targeted proteins will be selected jointly by ARS Beltsville and other members of the consortium. Selected recombinant antibodies will be provided to cooperators. Genes encoding the recombinant antibodies will also be available as needed. The Cooperator will identify candidate psyllid encoded proteins for screening and use the recombinant antibodies to test for inhibition of acquisition/transmission of the pathogen by Diaphorina citri.