Grant Information

EFFECT OF NUTRITIONAL SPRAY PROGRAMS ON HUANGLONGBING (HLB) DISEASE-INDUCED OFF-FLAVOR OF ORANGE FRUIT AND JUICE.

Sponsoring Institution Agricultural Research Service/USDA
Status ACTIVE
Funding Source USDA INHOUSE
Reporting Frequency Annual
Project Director BALDWIN E A
Accession Number 424029
Project Number 6034-41430-005-22R
Dates 2012-12-13 - 2015-01-31
Recipient Organization AGRICULTURAL RESEARCH SERVICE
219 SOUTH ROCK ROAD
FT PIERCE,FL 34945
Keywords (hlb)
citrus
disease
electronic
flavor
huanglongbing
juice
nose
orange
sensory
tongue
Research Effort Applied (40%)
Basic (40%)
Developmental (20%)
Classification Parameters
Knowledge AreaSubject of InvestigationField of SciencePercent
502 - New and Improved Food Products 999 - Citrus, general/other 1000 - Biochemistry and biophysics 50%
502 - New and Improved Food Products 1129 - Berries and cane fruits, general/other 1160 - Pathology 20%
503 - Quality Maintenance in Storing and Marketing Food Products 1469 - Solanaceous and related crops, general/other (for potato use 1310) 2000 - Chemistry 20%
503 - Quality Maintenance in Storing and Marketing Food Products 1099 - Tropical/subtropical fruit, general/other 1020 - Physiology 10%
Goals / Objectives
Evaluate juice quality from orange fruit (juice) harvested from groves treated with conventional spray programs compared to groves receiving various nutritional spray programs and determine components of off-flavors.
Methods (unparsed)

Harvest fruit symptomatic and asymptomatic for Huanglongbing (HLB) disease from trees receiving conventional spray programs and various nutritional spray programs for comparison to healthy fruit/juice, as well as recover oil and determine quality by measuring chemical components along with sensory panels. When off-flavors are detected, determine causal components and establish flavor thresholds for these components using gas-chromatography-mass spectroscopy (GC-MS), electronic nose and tongue, high performance liquid chromatography (HPLC) ¿ MS, and various types of sensory panels.

Methods
Harvest fruit symptomatic and asymptomatic for Huanglongbing (HLB) disease from trees receiving conventional spray programs and various nutritional spray programs for comparison to healthy fruit/juice, as well as recover oil and determine quality by measuring chemical components along with sensory panels. When off-flavors are detected, determine causal components and establish flavor thresholds for these components using gas-chromatography-mass spectroscopy (GC-MS), electronic nose and tongue, high performance liquid chromatography (HPLC) ' MS, and various types of sensory panels.
Project Timeline Tracking

Outputs

Evaluate juice quality from orange fruit (juice) harvested from groves treated with conventional spray programs compared to groves receiving various nutritional spray programs and determine components of off- flavors. Harvest fruit symptomatic and asymptomatic for Huanglongbing (HLB) disease from trees receiving conventional spray programs and various nutritional spray programs for comparison to healthy fruit/juice, as well as recover oil and determine quality by measuring chemical components along with sensory panels. When off-flavors are detected, determine causal components and establish flavor thresholds for these components using gas-chromatography-mass spectroscopy (GC-MS), electronic nose and tongue, high performance liquid chromatography (HPLC) � MS, and various types of sensory panels. This project is related to Objective 2 of the in-house project: Relate chemical composition to sensory flavor and pathogen resistance data to determine which compounds are important for flavor or have antimicrobial properties. Two Hamlin and two Valencia harvests were conducted for fruit from healthy and Huanglongbing (HLB) infected trees. Fruit were processed under simulated commercial conditions and samples frozen for sugar, acid, aroma volatiles, bitter limonoids, flavonoids, real time polymerase chain reaction (PCR) for deoxyribonucleaic acid (DNA), and sensory analyses. The chemical, biological and sensory analyses are underway.