Sponsoring Institution
Agricultural Research Service/USDA
Status
COMPLETE
Funding Source
USDA COOPERATIVE AGREEMENT
Reporting Frequency
Annual
Project Director
MANGAN R L
Accession Number
421036
Project Number
6204-43000-015-06S
Agreement Number
421036
Dates
2011-02-01 - 2012-06-30
Recipient Organization
TEXAS A&M UNIVERSITY - KINGSVILLE CITRUS CENTER
312 N. INTERNATIONAL BLVD.
WESLACO,TX 78596
Keywords
asian
citrus
huanglongbing
psyllid
Research Effort
Applied (50%)
Basic (25%)
Developmental (25%)
Classification Parameters
| Knowledge Area | Subject of Investigation | Field of Science | Percent |
|---|
| 211 - Insects, Mites, and Other Arthropods Affecting Plants |
910 - Grapefruit |
1130 - Entomology and acarology |
50% |
| 211 - Insects, Mites, and Other Arthropods Affecting Plants |
920 - Orange |
1130 - Entomology and acarology |
50% |
Goals / Objectives
The objective of this cooperative research project is to survey insect vectors and plant tissues for the presence of Huanglongbing citrus disease in Rio Grande Valley, manage the USDA certified detection laboratory and develop more efficient, cheaper and more accurate methods for detecting HLB in plant and insect tissues.
Methods (unparsed)
Individual samples of citrus leaf and Asian Citrus Psyllids are analyzed using PCR molecular techniques. The technique currently assigns risk scores, and suspect samples are forwarded to USDA laboratories in Maryland for further analysis. Research to improve the current technique will focus on reducing the time between infection and its detection in plant tissues, and the reduction in false positives in leaf tissue. Techniques other than PCR analysis will be explored.
Methods
Individual samples of citrus leaf and Asian Citrus Psyllids are analyzed using PCR molecular techniques. The technique currently assigns risk scores, and suspect samples are forwarded to USDA laboratories in Maryland for further analysis. Research to improve the current technique will focus on reducing the time between infection and its detection in plant tissues, and the reduction in false positives in leaf tissue. Techniques other than PCR analysis will be explored.
Project Timeline Tracking
Outputs
FY12 Appropriation directed closure of Unit; project officially terminated 6/30/12. Limited research was conducted during FY12; all efforts were directed toward the efficient finalization of this project. Cooperation involved co-authorship of manuscripts, and periodic updates and a written annual report were made.