Grant Information

EVALUATION OF HLB/ACP RESISTANCE IN PONCIRUS TRIFOLIATA HYBRIDS AND ASSESSMENT OF DNA MARKERS AND POSSIBLE RESISTANCE GENES

Sponsoring Institution Agricultural Research Service/USDA
Status ACTIVE
Funding Source USDA INHOUSE
Reporting Frequency Annual
Project Director STOVER E W
Accession Number 420682
Project Number 6034-21000-014-02N
Agreement Number 420682
Dates 2010-12-01 - 2015-09-30
Recipient Organization UNIVERSITY OF FLORIDA

LAKE ALFRED,FL 33850
Keywords asian
assisted
citrus
huanglongbing
marker
psyllid
resistance
selection
transformation
Research Effort Applied (50%)
Basic (50%)
Developmental (0%)
Classification Parameters
Knowledge AreaSubject of InvestigationField of SciencePercent
204 - Plant Product Quality and Utility (Preharvest) 920 - Orange 1080 - Genetics (excludes breeding) 40%
203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants 910 - Grapefruit 1000 - Biochemistry and biophysics 30%
212 - Pathogens and Nematodes Affecting Plants 999 - Citrus, general/other 1160 - Pathology 30%
Goals / Objectives
Evaluate array of Poncirus hybrids and standard cultivars to ultimately identify which genes are associated for use in conventional and transgenic plant improvement.
Methods (unparsed)

Plant replicated trial with 8 plants of each of numerous citranges (from a well-characterized mapping population), other poncirus hybrids and standard cultivars. Material will be at USDA/ARS Picos Farm where HLB and ACP are rampant and will be screened in response to natural exposure.

Methods
Plant replicated trial with 8 plants of each of numerous citranges (from a well-characterized mapping population), other poncirus hybrids and standard cultivars. Material will be at USDA/ARS Picos Farm where HLB and ACP are rampant and will be screened in response to natural exposure.
Project Timeline Tracking

Outputs

This project is related to Objective 1: Create new genetic combinations of citrus, Objective 2: Screen germplasm for important traits and select superior individuals, Sub objective 1 D: Create new scions and rootstocks with potential resistance to huanglongbing (HLB) and citrus bacterial canker (CBC) by genetic transformation. More than 120 citranges, from a well-characterized mapping population, and other trifoliate hybrids (+ sweet orange standards) have been planted in a replicated trial in collaboration with University of Florida and University of California/Riverside. Plants are being monitored for Liberibacter development and HLB symptoms. A USDA/ARS advanced selection which is 1/16th Poncirus, with commercial quality fruit, is performing extremely well. Data from this trial should provide information on markers and perhaps genes associated with huanglongbing (HLB) resistance, for use in transgenic and conventional breeding. The University of California-Riverside has genotyped these plants by sequencing.