Grant Information

COMBINATORIAL LIBRARY SCREENING FOR PSYLLID DISRUPTION MOLECULES

Sponsoring Institution Agricultural Research Service/USDA
Status ACTIVE
Funding Source USDA COOPERATIVE AGREEMENT
Reporting Frequency Annual
Project Director SHATTERS R G
Accession Number 420127
Project Number 6618-22320-001-17S
Dates 2010-05-01 - 2011-03-31
Recipient Organization Torrey Pines Institute for Molecular Studies
11350 SW Village Parkway
Port St. Lucie,FL 34981-2352
Keywords asian
citrus
greening
hlb
huanglongbing
peptides
psyllid
Research Effort Applied (30%)
Basic (45%)
Developmental (25%)
Classification Parameters
Knowledge AreaSubject of InvestigationField of SciencePercent
215 - Biological Control of Pests Affecting Plants 2110 - Ornamental trees and shrubs 1130 - Entomology and acarology 20%
211 - Insects, Mites, and Other Arthropods Affecting Plants 2122 - Potted plants 1130 - Entomology and acarology 15%
211 - Insects, Mites, and Other Arthropods Affecting Plants 920 - Orange 1130 - Entomology and acarology 15%
216 - Integrated Pest Management Systems 1460 - Tomato 1130 - Entomology and acarology 15%
216 - Integrated Pest Management Systems 910 - Grapefruit 1130 - Entomology and acarology 15%
211 - Insects, Mites, and Other Arthropods Affecting Plants 1499 - Vegetables, general/other 1130 - Entomology and acarology 10%
215 - Biological Control of Pests Affecting Plants 1429 - Cucurbits, other (includes pumpkin, squash, gourd) 1130 - Entomology and acarology 10%
Goals / Objectives
To identify novel compounds that can be used for controlling the Asian citrus psyllid.
Methods (unparsed)

Synthesize and provide combinatorial libraries that will be used by USDA ARS U.S. Horticultural Research Laboratory researchers in psyllid artificial diet bioassays.

Methods
Synthesize and provide combinatorial libraries that will be used by USDA ARS U.S. Horticultural Research Laboratory researchers in psyllid artificial diet bioassays.
Project Timeline Tracking

Outputs

This project is related inhouse project objective 3b: Develop novel control methods based on disrupting key processes in pest biology. Second round screening of peptides that blocked Asian citrus psyllid (ACP) feeding using an artificial diet bioassay have led to verification of peptide pools from a combinatorial library that have a significant effect on both ACP feeding and survival. Work is continuing to identify the active peptides within these pools. Furthermore, specific proteins were shown to block Asian citrus psyllid feeding on citrus leaves when these proteins were applied to the surface of the leaf before psyllid feeding was initiated.